Effect of three extraction methods on yield, antioxidant activity, total phenols, and stability of extracts from Plectranthus amboinicus leaves
DOI:
https://doi.org/10.29059/cienciauat.v18i2.1797Keywords:
Oreganon, extraction, antioxidant activity, phenolic compounds, yieldAbstract
Conventional phytochemicals extraction methods have shown low yields or very long processing times for obtaining. The ultrasound offers alternatives to increase the yields of plant extracts. The objective of this work was to evaluate the effect of three extraction methods on the yield, phenolic compound composition, antioxidant activity and stability of Plectranthus amboinicus (oregano) leaf extracts. The extraction methods used were: conventional heat-assisted extraction (CE), ultrasound water bath assisted (UBAE) and ultrasound probe assisted extraction (UPAE). A 1:10 (w/v) oregano: ethanol ratio was used at 35 ± 2 °C. Yields were calculated as extract dry matter (% ms). A pseudo-second-order model was used to fit the experimental extraction curves of the three methods and obtain the kinetic parameters. Total phenolic compounds (TPC) and antioxidant activity (AA) were determined by Folin-Ciocalteau and DPPH methods, respectively. The stability of TPC and AA was evaluated during storage. UPAE increased 33.93 % yield in extracts concerning CE. The highest concentration of CFT (P ≤ 0.05) was obtained by UBAE (10.32 mg EAG/ms) and for AA by CE (19.43 mg ET/g ms). The pseudo-second-order kinetic model adequately fitted the experimental kinetics. The highest TPC rate constant (k) was 0.016 g ms/mg EAG·min, for UPAE, this technique being the one that demonstrated a significant decrease in AA with respect to extraction time. The TPC of the extracts obtained by UBAE, UPAE and CE (3.7 mg EAG/g dms, 4.4 mg EAG/g dms and 7.2 mg EAG/g ms, respectively), as well as the AA remained stable and without significant changes (P ≥ 0.05) during the 34 d of storage, again showing the advantage of using the ultrasound extractive technique, as a function of time.
References
Abubakar, A. R. and Haque, M. (2020). Preparation of medicinal plants: basic extraction and fractionation procedures for experimental purposes. Journal of Pharmacy & BioAllied Sciences. 12(1): 1-10.
Acosta-Martínez, D. R., Morales-Zamora, M. y Villanueva-Ramos, G. (2021). Obtención de extracto fluido de Plectranthus amboinicus (orégano), utilizando el método de agitación mecánica. Afinidad. 78(592): 41-47.
Agusti, B. A., Mansur, M. M., and Linde, A. M. (2014). Technical evaluation of antioxidant activity. Medicinal Chemistry. 4(7): 517-522.
Avello, M. y Suwalsky, M. (2006). Radicales libres, antioxidantes naturales y mecanismos de protección. Atenea (Concepción). (494): 161-172.
Azwanida, N. A. (2015). Review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants. 4(3): 1-6.
Bañuelos-Hernández, A. E., Azadniya, E., Ramírez, M. E., and Morlock, G. E. (2020). Bioprofiling of mexican Plectranthus amboinicus (Lour.) essential oil via planar chromatography-effect-directed analysis combined with direct analysis in real time high-resolution mass spectrometry. Journal of Liquid Chromatography & Related Technologies. 43(9-10): 344-350.
Bendicho, C., De-La-Calle, I., Pena, F., Costas, M., Cabaleiro, N., and Lavilla, I. (2012). Ultrasound-assisted pretreatment of solid samples in the context of green analytical chemistry. Trends in Analytical Chemistry. 31: 50-60.
Benítez, B., Sarria, R., Gallo, J., Pacheco, N., Sandoval, J. y Aristizabal, C. (2019). Obtención y rendimiento del extracto etanólico de dos plantas medicinales. Revista Facultad de Ciencias Básicas. 15(1): 31-40.
Bhagya, G. V. S. and Dash, K. K. (2020). Ultrasound-assisted extraction of phytocompounds from dragon fruit peel: Optimization, kinetics and thermodynamic studies. Ultrasonic Sonochemistry. 68: 105180.
Bhave, A. and Dasgupta, S. (2018). Effect of cooking on total phenol, total flavonoids and DPPH free radical scavenging potential of Plectranthus amboinicus. Journal of Medicinal Plants Studies. 6(3): 82-84.
Corona-Jiménez, E., Martínez-Navarrete, N., Ruiz-Espinosa, H. y Carranza-Concha, J. (2016). Extracción asistida por ultrasonido de compuestos fenólicos de semillas de chía (Salvia hispánica L.) y su actividad antioxidante. Agrociencia. 50(3): 403-412.
El-hawary, S. S., El-sofanya, R. H., Abdel-Monema, A. R., Ashoura, R. S., and Sleem, A. A. (2012). Polyphenolics content and biological activity of Plectranthus amboinicus (Lour.) Spreng growing in Egypt (Lamiaceae). Pharmacognosy Journal. 4(32): 45-54.
Ercoli, S., Cartes, J., Cornejo, P., Tereucán, G., Winterhalter, P., Contreras, B., and Ruiz, A. (2021). Stability of phenolic compounds, antioxidant activity and colour parameters of a coloured extract obtained from coloured-flesh potatoes. LWT - Food Science and Technology. 136: 110370.
Galiano, D. (2019). Determinación de la capacidad antioxidante de Thymus vulgaris. España: Universidad de Jaén. 49 Pp.
Gordo, D. (2018). Los compuestos fenólicos: un acercamiento a su biosíntesis, síntesis y actividad biológica. Revista de Investigación Agraria y Ambiental. 9(1): 81-104.
Isah, T. (2019). Stress and defense responses in plant secondary metabolites production. Biological Research. 52(39).
Koztowska, M., Scibisz, I., Przybyl, J. L., Ziarno, M., Zbikowska, A., and Majewska, E. (2021). Phenolic contents and antioxidant activity of extracts of selected fresh and dried herbal materials. Polish Journal of Food and Nutrition Sciences. 71(3): 269-278.
Kulbat-Warycha, K., Oracz, J., and Zyzelewicz, D. (2022). Bioactive properties of extracts from Plectranthus barbatus (Coleus forskohlii) roots received using various extraction methods. Molecules. 27(24): 8986.
Kumar, S., Azam M., Venkatesan K., Anjly P., and Kulloli R. (2019). Morphological and Biochemical Variability in Aloe Germplasm in Hot Arid Region of India. Journal of Herbs, Spices & Medicinal Plants. 25(2): 158-171.
Kumar, P., Sangam, and Kumar, N. (2020). Plectranthus amboinicus: A review on its pharmacological and pharmacognostical studies. American Journal of Physiology, Biochemistry and Pharmacology. 10(2): 55-62.
López, S. M., Triana, J., Pérez, F. J. y Torres, M. E. (2005). Métodos físicos de separación y purificación de sustancias orgánicas. España: Universidad de las Palmas de Gran Canaria. 52 Pp.
Matias, D., Nicolai, M., Fernandes, A. S., Saraiva, N., Almeida, J., Saraiva, L. and Rijo, P. (2019). Comparison study of different extracts of Plectranthus madagascariensis, P. neochilus and the rare P. porcatus (Lamiaceae): chemical characterization, antioxidant, antimicrobial and cytotoxic activities. Biomolecules. 9(5): 179.
Menéndez, R. A. y Pavón, V. (1999). Plectranthus amboinicus (Lour.) Spreng. Revista Cubana de Plantas Medicinales. 4(3): 110-115.
M’hiri, N., Ioannou, I., Boudhrioua, N. M., and Ghoul, M. (2015). Effect of different operating conditions on the extraction of phenolic compounds in orange peel. Food and Bioproducts Processing. 96: 161-170.
Moreno, J., López, G. y Siche, R. (2010). Modelación y optimización del proceso de extracción de aceite esencial de eucalipto (Eucalyptus globulus). Scientia Agropecuaria. 1(2): 147-154.
Morton, J. F. (1992). Country horage (Coleus amboinicus Lour) A potent flavoring and medicinal plant. Journal Herbs Spices Medicinal Plants. 1(1-2): 77-90.
Muhamad, S. and Mat, A. S. F. Z. (2018). Effect of heating at 95°C on antioxidant activity and total phenolic content in extracts of Plectranthus amboinicus leaves. International Journal of Recent Scientific Research. 9(7): 28096-28099.
Muhamad, S. and Mat, A. S. F. Z. (2019). Boiling increase antioxidant activity, total phenolic content and total flavonoid content in Plectranthus amboinicus leaves. GSC Biological and Pharmaceutical Sciences. 6(3): 024-030.
Nguyen, N. Q., Minh, L. V., Trieu, L. H., Bui, L. M., Lam, T. D., Hieu, V. Q., …, and Trung, L. N. Y. (2020). Evaluation of total polyphenol content, total flavonoid content, and antioxidant activity of Plectranthus amboinicus leaves. Energy Security and Chemical Engineering Congress. Conf. Series: Materials Science and Engineering. 736(6): 062017.
Nurafifah, F., Luqman, C. A., and Puteri, F. W. M. A. (2018). Drying of Plectranthus amboinicus (Lour) spreng leaves by using oven dryer. Engineering in Agriculture, Environment and Food. 11(4): 239-244.
Palomino, G. L. R., García, P. C. M., Gil, G. J. H., Rojano, B. A. y Durango, R. D. L. (2009). Determinación del contenido de fenoles y evaluación de la actividad antioxidante de propóleos recolectados en el departamento de Antioquía (Colombia). Universidad de Antioquía Medellín, Colombia. 16(3): 388-395.
Pashazadeh, H., Zannou, O., Galanakis, C. M., Al-dawoud, T. M. S., Ibrahim, S. A., and Koca, I. (2021). Optimization of drying process for Rosa pimpinellifolia L. (black rose hips) based on bioactive compounds and modeling of drying process. International Journal of Food Properties. 24(1): 1367-1386.
Patel, R. D., Mahobia, N. K., Singh, M. P., Singh, A., Sheikh, N. W., Alam, G., and Singh, S. K. (2010). Antioxidant potential of leaves of Plectranthus amboinicus (Lour) Spreng. Der Pharmacia Lettre. 2(4): 240-245.
Pingret, D., Fabiano-Tixier, A. S., Le-Bourvellec, C., Renard, C. M., and Chemat, F. (2012). Lab and pilot-scale ultrasound-assisted water extraction of polyphenols from apple pomace. Journal of Food Engineering. 111(1): 73-81.
Prasad, N., Basalingappa, K. M., Gopenath, T. S., Razvi, S. M., Murugesan, K., Ashock, G., and Divyashree, K. C. (2020). Nutritional significance of indian borage (Plectranthus amboinicus): A review. Plant Archives. 20(2): 3727-3730.
Ramírez-Reyes, T., Luna-Rodríguez, M., Noa-Carrazana, J. C., Díaz-Fleischeer, F., Sánchez-Velásquez, L. R., and Flores-Estévez, N. (2015). Influence of season and organ on antibacterial activity of Magnolia dealbata Zucc. Against two phytopathogenic bacteria. Chemistry and Ecology. 31(1): 47-52.
Rivas-Pérez, B. N., Leal-Granadillo, I. A., Loaiza-Cuauro, L. F., Morillo, Y. E., and Colina-Chirinos, J. C. (2017). Phenolic compounds and antioxidant activity in extracts of four oregano spices. Revista Técnica de la Facultad de Ingeniería Universidad de Zulia. 40(3): 134-142.
Ruiz-de-Anda, D., Ventura-Lara, M. G., Rodríguez-Hernández, G., and Ozuna, C. (2019). The impact of power ultrasound application on physicochemical, antioxidant, and microbiological properties of fresh orange and celery juice blend. Journal of Food Measurement and Characterization. 13: 3140-3148.
Sabra, A. S., Astatkie, T., Alataway, A., Mahmoud, A. A., Gendy, A. S. H., Said-Al-Ahl, H. A., and Tkachenko, K. G. (2018). Response of biomass development, essential oil, and composition of Plectranthus amboinicus (Lour.) Spreng. to irrigation frequency and harvest time. Chemistry & Biodiversity. 15(3): e1800005.
Samad, N. A., Abang-Zaidel, D. N., Salleh, E., Mohd-Yusof, A. H., Dailin, D. J., and Abang-Zaide, D. N. (2019). Optimization of Plectranthus amboinicus (Lour.) Spreng extraction process using microwave-assisted technique. Chemical Engineering Transactions. 72: 397-402.
Silalahi, M. (2018). Plectranthus amboinicus (lour.) Spreng sebagai bahan pangan dan obat serta bioaktivitasnya. Journal Dinamika Pendidikan. 11(2): 123-138.
Singh-Sengar, A., Rawson, A., Muthiah, M., and Kumar-Kalakandan, S. (2020). Comparison of different ultrasound assisted extraction techniques for pectin from tomato processing waste. Ultrasonic Sonochemistry. 61: 104812.
Shubha, J. R. and Bhatt, P. (2015). Plectranthus amboinicus leaves stimulate growth of probiotic L. plantarum: Evidence for ethnobotanical use in diarrhea. Journal of Ethnopharmacology. 166: 220-227.
Tungmunnithum, D., Garros, L., Drouet, S., Renouard, S., Lainé, E., and Hano, C. (2019). Green ultra-sound-assisted extraction of trans rosmarinic acid from Plectranthus scutellarioides (L.) R. Br. leaves. Plants. 8(3): 50.
Vasco, C., Ruales, J., and Kamal-Eldin, A. (2008). Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chemistry. 111: 816-823.
Vilaplana, M. (2007). Antioxidantes presentes en los alimentos: vitaminas, minerales y suplementos. Ámbito Farmacéutico Nutrición. 26(10): 79-86.
Wibisono, K., Aisyah, S. I., Suhesti, S., and Nurcholis, W. (2019). Optimization of total flavonoids extraction and A-glucosidase inhibitory activity from Plectranthus amboinicus (Lour.) Spreng. Leaves using the simplex-centroid design. Molekul. 14(2): 84-91.
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Universidad Autónoma de Tamaulipas
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
