Wound healing effect induced by Mangifera indica var. manila proteins in human fibroblast
DOI:
https://doi.org/10.29059/cienciauat.v20i1.1972Keywords:
wound healing, human fibroblasts, mango proteins, cell migration, essay wound scarAbstract
Ayurvedic medicine has reported the use of Mangifera indica L (mango) to heal wounds caused by stings and bites from certain animals. The aim of this study was to evaluate the healing effects of mango proteins in vitro in a wound scar model in human fibroblasts. The total proteins from mango pulp were isolated and quantified using the Bradford method and subsequently the samples were lyophilized and quantified by BCA. SDS-PAGE electrophoresis was used for the separation and identification of the protein fractions. Assays to determine protein cytotoxicity were performed by quantifying fibroblast viability at different protein concentrations (0.25 mg/mL, 2.5 mg/mL, and 25 mg/mL). The scratch wound assay was carried out through scraping the fibroblast culture, with wound length being measured via microscopy after treatment with the different protein concentrations at 24 h, and 48 h and the percentage of wound closure being calculated. The isolated mango proteins had a molecular weight between 10 kDa and 95 kDa and similar to the control (P < 0.05) they did not induce cytotoxicity or morphological changes in fibroblasts at the concentrations used. The treatment using a 0.25 mg/mL protein suggests an increase in fibroblast migration that could favor wound closure.
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